Spatial mapping of extracellular oxidant production by a white rot basidiomycete on wood reveals details of ligninolytic mechanism.
Identifieur interne : 000361 ( Main/Exploration ); précédent : 000360; suivant : 000362Spatial mapping of extracellular oxidant production by a white rot basidiomycete on wood reveals details of ligninolytic mechanism.
Auteurs : Christopher G. Hunt [États-Unis] ; Carl J. Houtman ; Don C. Jones ; Peter Kitin ; Premsagar Korripally ; Kenneth E. HammelSource :
- Environmental microbiology [ 1462-2920 ] ; 2013.
Descripteurs français
- KwdFr :
- MESH :
- biosynthèse : Oxydants.
- composition chimique : Alcools benzyliques, Phanerochaete.
- génétique : Phanerochaete.
- microbiologie : Bois.
- métabolisme : Hyphae, Lignine, Oxydants, Phanerochaete.
- Période.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : Oxidants.
- chemical , chemistry : Benzyl Alcohols.
- chemistry : Phanerochaete.
- genetics : Phanerochaete.
- metabolism : Hyphae, Lignin, Oxidants, Phanerochaete.
- microbiology : Wood.
- Half-Life.
Abstract
Oxidative cleavage of the recalcitrant plant polymer lignin is a crucial step in global carbon cycling, and is accomplished most efficiently by fungi that cause white rot of wood. These basidiomycetes secrete many enzymes and metabolites with proposed ligninolytic roles, and it is not clear whether all of these agents are physiologically important during attack on natural lignocellulosic substrates. One new approach to this problem is to infer properties of ligninolytic oxidants from their spatial distribution relative to the fungus on the lignocellulose. We grew Phanerochaete chrysosporium on wood sections in the presence of oxidant-sensing beads based on the ratiometric fluorescent dye BODIPY 581/591. The beads, having fixed locations relative to the fungal hyphae, enabled spatial mapping of cumulative extracellular oxidant distributions by confocal fluorescence microscopy. The results showed that oxidation gradients occurred around the hyphae, and data analysis using a mathematical reaction-diffusion model indicated that the dominant oxidant during incipient white rot had a half-life under 0.1 s. The best available hypothesis is that this oxidant is the cation radical of the secreted P. chrysosporium metabolite veratryl alcohol.
DOI: 10.1111/1462-2920.12039
PubMed: 23206186
Affiliations:
Links toward previous steps (curation, corpus...)
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Jones</name></author><author><name sortKey="Kitin, Peter" sort="Kitin, Peter" uniqKey="Kitin P" first="Peter" last="Kitin">Peter Kitin</name></author><author><name sortKey="Korripally, Premsagar" sort="Korripally, Premsagar" uniqKey="Korripally P" first="Premsagar" last="Korripally">Premsagar Korripally</name></author><author><name sortKey="Hammel, Kenneth E" sort="Hammel, Kenneth E" uniqKey="Hammel K" first="Kenneth E" last="Hammel">Kenneth E. Hammel</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23206186</idno><idno type="pmid">23206186</idno><idno type="doi">10.1111/1462-2920.12039</idno><idno type="wicri:Area/Main/Corpus">000388</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000388</idno><idno type="wicri:Area/Main/Curation">000388</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000388</idno><idno type="wicri:Area/Main/Exploration">000388</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Spatial mapping of extracellular oxidant production by a white rot basidiomycete on wood reveals details of ligninolytic mechanism.</title><author><name sortKey="Hunt, Christopher G" sort="Hunt, Christopher G" uniqKey="Hunt C" first="Christopher G" last="Hunt">Christopher G. 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Jones</name></author><author><name sortKey="Kitin, Peter" sort="Kitin, Peter" uniqKey="Kitin P" first="Peter" last="Kitin">Peter Kitin</name></author><author><name sortKey="Korripally, Premsagar" sort="Korripally, Premsagar" uniqKey="Korripally P" first="Premsagar" last="Korripally">Premsagar Korripally</name></author><author><name sortKey="Hammel, Kenneth E" sort="Hammel, Kenneth E" uniqKey="Hammel K" first="Kenneth E" last="Hammel">Kenneth E. Hammel</name></author></analytic><series><title level="j">Environmental microbiology</title><idno type="eISSN">1462-2920</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Benzyl Alcohols (chemistry)</term><term>Half-Life (MeSH)</term><term>Hyphae (metabolism)</term><term>Lignin (metabolism)</term><term>Oxidants (biosynthesis)</term><term>Oxidants (metabolism)</term><term>Phanerochaete (chemistry)</term><term>Phanerochaete (genetics)</term><term>Phanerochaete (metabolism)</term><term>Wood (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alcools benzyliques (composition chimique)</term><term>Bois (microbiologie)</term><term>Hyphae (métabolisme)</term><term>Lignine (métabolisme)</term><term>Oxydants (biosynthèse)</term><term>Oxydants (métabolisme)</term><term>Phanerochaete (composition chimique)</term><term>Phanerochaete (génétique)</term><term>Phanerochaete (métabolisme)</term><term>Période (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Oxidants</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Benzyl Alcohols</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Oxydants</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Alcools benzyliques</term><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Hyphae</term><term>Lignin</term><term>Oxidants</term><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Bois</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Wood</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Hyphae</term><term>Lignine</term><term>Oxydants</term><term>Phanerochaete</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Half-Life</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Période</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Oxidative cleavage of the recalcitrant plant polymer lignin is a crucial step in global carbon cycling, and is accomplished most efficiently by fungi that cause white rot of wood. These basidiomycetes secrete many enzymes and metabolites with proposed ligninolytic roles, and it is not clear whether all of these agents are physiologically important during attack on natural lignocellulosic substrates. One new approach to this problem is to infer properties of ligninolytic oxidants from their spatial distribution relative to the fungus on the lignocellulose. We grew Phanerochaete chrysosporium on wood sections in the presence of oxidant-sensing beads based on the ratiometric fluorescent dye BODIPY 581/591. The beads, having fixed locations relative to the fungal hyphae, enabled spatial mapping of cumulative extracellular oxidant distributions by confocal fluorescence microscopy. The results showed that oxidation gradients occurred around the hyphae, and data analysis using a mathematical reaction-diffusion model indicated that the dominant oxidant during incipient white rot had a half-life under 0.1 s. The best available hypothesis is that this oxidant is the cation radical of the secreted P. chrysosporium metabolite veratryl alcohol.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">23206186</PMID><DateCompleted><Year>2013</Year><Month>11</Month><Day>26</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1462-2920</ISSN><JournalIssue CitedMedium="Internet"><Volume>15</Volume><Issue>3</Issue><PubDate><Year>2013</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Environmental microbiology</Title><ISOAbbreviation>Environ Microbiol</ISOAbbreviation></Journal><ArticleTitle>Spatial mapping of extracellular oxidant production by a white rot basidiomycete on wood reveals details of ligninolytic mechanism.</ArticleTitle><Pagination><MedlinePgn>956-66</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/1462-2920.12039</ELocationID><Abstract><AbstractText>Oxidative cleavage of the recalcitrant plant polymer lignin is a crucial step in global carbon cycling, and is accomplished most efficiently by fungi that cause white rot of wood. These basidiomycetes secrete many enzymes and metabolites with proposed ligninolytic roles, and it is not clear whether all of these agents are physiologically important during attack on natural lignocellulosic substrates. One new approach to this problem is to infer properties of ligninolytic oxidants from their spatial distribution relative to the fungus on the lignocellulose. We grew Phanerochaete chrysosporium on wood sections in the presence of oxidant-sensing beads based on the ratiometric fluorescent dye BODIPY 581/591. The beads, having fixed locations relative to the fungal hyphae, enabled spatial mapping of cumulative extracellular oxidant distributions by confocal fluorescence microscopy. The results showed that oxidation gradients occurred around the hyphae, and data analysis using a mathematical reaction-diffusion model indicated that the dominant oxidant during incipient white rot had a half-life under 0.1 s. The best available hypothesis is that this oxidant is the cation radical of the secreted P. chrysosporium metabolite veratryl alcohol.</AbstractText><CopyrightInformation>Published 2012. This article is a U.S. Government work and is in the public domain in the USA.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hunt</LastName><ForeName>Christopher G</ForeName><Initials>CG</Initials><AffiliationInfo><Affiliation>US Forest Products Laboratory, Madison, WI 53726, USA. cghunt@fs.fed.us</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Houtman</LastName><ForeName>Carl J</ForeName><Initials>CJ</Initials></Author><Author ValidYN="Y"><LastName>Jones</LastName><ForeName>Don C</ForeName><Initials>DC</Initials></Author><Author ValidYN="Y"><LastName>Kitin</LastName><ForeName>Peter</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Korripally</LastName><ForeName>Premsagar</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Hammel</LastName><ForeName>Kenneth E</ForeName><Initials>KE</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>12</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Environ Microbiol</MedlineTA><NlmUniqueID>100883692</NlmUniqueID><ISSNLinking>1462-2912</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001592">Benzyl Alcohols</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016877">Oxidants</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>MB4T4A711H</RegistryNumber><NameOfSubstance UI="C042197">veratryl alcohol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001592" MajorTopicYN="N">Benzyl Alcohols</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006207" MajorTopicYN="N">Half-Life</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D025301" MajorTopicYN="N">Hyphae</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016877" MajorTopicYN="N">Oxidants</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>10</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>11</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>12</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>12</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>12</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23206186</ArticleId><ArticleId IdType="doi">10.1111/1462-2920.12039</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Wisconsin</li></region></list><tree><noCountry><name sortKey="Hammel, Kenneth E" sort="Hammel, Kenneth E" uniqKey="Hammel K" first="Kenneth E" last="Hammel">Kenneth E. Hammel</name><name sortKey="Houtman, Carl J" sort="Houtman, Carl J" uniqKey="Houtman C" first="Carl J" last="Houtman">Carl J. Houtman</name><name sortKey="Jones, Don C" sort="Jones, Don C" uniqKey="Jones D" first="Don C" last="Jones">Don C. Jones</name><name sortKey="Kitin, Peter" sort="Kitin, Peter" uniqKey="Kitin P" first="Peter" last="Kitin">Peter Kitin</name><name sortKey="Korripally, Premsagar" sort="Korripally, Premsagar" uniqKey="Korripally P" first="Premsagar" last="Korripally">Premsagar Korripally</name></noCountry><country name="États-Unis"><region name="Wisconsin"><name sortKey="Hunt, Christopher G" sort="Hunt, Christopher G" uniqKey="Hunt C" first="Christopher G" last="Hunt">Christopher G. Hunt</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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